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KMID : 1134820160450111589
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 11 p.1589 ~ p.1594
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Induction of Apoptosis and Inhibition of Growth in Human Gastric Cancer by Piperine
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Shin Seong-Ah
Lee Hae-Nim
Choo Gang-Sik
Kim So-Jung
Kim Hyeong-Jin
Park Young-Suk
Park Byung-Kwon
Kim Byeong-Soo
Kim Sang-Ki
Lee Hu-Jang
Jung Ji-Youn
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Abstract
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Piperine [(E,E)-5-(3,4-methtylenedioxyphenyl)-2,4-pentadienolypiperidide] is a principal of Piperaceae, including Piper nigrum L. and Piper longum Linn., which has been used as a spice and traditional medicine. In this study, we investigated whether or not piperine has anti-cancer effects on AGS human gastric cancer cells. The results demonstrated that piperine not only inhibited proliferation using MTT assay but also induced apoptotic bodies using DAPI assay in a dose-dependent manner in response to piperine. Expression levels of p53, Bax (pro-apoptotic), cleaved caspase-9, and cleaved-PARP ?increased, whereas expression levels of Bcl-2, XIAP (anti-apoptotic), and Akt decreased in a dose-dependent manner compared with the control by western blotting analysis. To identify the connection between phospo-Akt and Bcl-2 family in response to piperine, LY249002 (Akt inhibitor) was treated with piperine (150 μM). The results were shown that expression of phospo-Akt was reduced whereas expression of Bax and cleaved-PARP increased in a dose-dependent manner. These results indicate that piperine induced apoptosis in AGS cells and may serve as a chemopreventive or therapeutic agent for human gastric cancer.
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KEYWORD
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cancer cell, apoptosis, anticancer, piperine, Akt signaling
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